Multiple internal controls enhance reliability for PCR and real time PCR detection of Rathayibacter toxicus.

ABSTRACT: Rathayibacter toxicus is a toxigenic bacterial plant pathogen indigenous to Australia and South Africa. A threat to livestock industries globally, the bacterium was designated a U.S. Select Agent. Biosecurity and phytosanitary concerns arise due to the international trade of seed and hay that harbor the bacterium. Accurate diagnostic protocols to support phytosanitary decisions, delineate areas of freedom, and to support research are required to address those concerns. Whole genomes of three genetic populations of R. toxicus were sequenced (Illumina MiSeq platforms), assembled and genomic regions unique to each population identified. Highly sensitive and specific TaqMan qPCR and multiplex endpoint PCR assays were developed for the detection and identification of R. toxicus to the populatio

SUBMITTER: Arif M 

PROVIDER: S-EPMC8052354 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature