Identification and characterization of alkaline phosphatase gene phoX in Microcystis aeruginosa PCC7806.

ABSTRACT: PhoX is an extracellular alkaline phosphatase that is widely found in cyanobacteria and plays an important role in the conversion of extracellular organophosphorus into soluble inorganic phosphorus. However, the phoX gene has not yet been experimentally confirmed to exist in bloom-forming Microcystis species. In this study, we identified a putative phoX gene (GenBank accession no. ARI79942.1) in M. aeruginosa PCC7806 and overexpressed it in Escherichia coli 21 (DE3). The expressed PhoX protein displayed phosphodiesterase and phosphomonoesterase activities. In contrast to other bacterial PhoX proteins, which are activated mainly by Ca²⁺, Microcysits PhoX was most strongly activated by Mg²⁺, followed by Co²⁺, Ca²

SUBMITTER: Hong S 

PROVIDER: S-EPMC8052394 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature