Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation.

ABSTRACT: In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and reproducible parameters. To address this challenge, a robust and scaffold-free approach is proposed, which relies on multi-trap acoustic levitation. This technology is successfully applied to Mesenchymal Stem Cells (MSCs) maintained in acoustic levitation over a 24-h period. During the culture, MSCs spontaneously self-organized from cell sheet

SUBMITTER: Jeger-Madiot N 

PROVIDER: S-EPMC8052426 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature