Silencing SHMT2 inhibits the progression of tongue squamous cell carcinoma through cell cycle regulation.

ABSTRACT:

Background

Serine hydroxymethyltransferase 2 (SHMT2) is a vital metabolic enzyme in one carbon metabolism catalyzing the conversion of serine to glycine, which has been reported to play a crucial role in the progression of tumors. However, its function in tongue squamous cell carcinoma (TSCC) remains unclear.

Methods

SHMT2 expression was analyzed using samples in online databases, and was assessed through immunohistochemistry staining of collected clinical specimens. The correlation between SHMT2 expression and the cell cycle was predicted through bioinformatic analysis, including weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA). After transfection with siRNA, CCK8 assay, Edu staining, flow cytometry, trans-well assay, and wound he

SUBMITTER: Liao Y 

PROVIDER: S-EPMC8052717 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature