In vivo phage display: identification of organ-specific peptides using deep sequencing and differential profiling across tissues.

ABSTRACT: In vivo phage display is widely used for identification of organ- or disease-specific homing peptides. However, the current in vivo phage biopanning approaches fail to assess biodistribution of specific peptide phages across tissues during the screen, thus necessitating laborious and time-consuming post-screening validation studies on individual peptide phages. Here, we adopted bioinformatics tools used for RNA sequencing for analysis of high-throughput sequencing (HTS) data to estimate the representation of individual peptides during biopanning in vivo. The data from in vivo phage screen were analyzed using differential binding-relative representation of each peptide in the target organ versus in a panel of control organs. Application of this approach in a model study using low-diversity

SUBMITTER: Pleiko K 

PROVIDER: S-EPMC8053097 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature