Two-step functional screen on multiple proteinaceous substrates reveals temperature-robust proteases with a broad-substrate range.

ABSTRACT: To support the bio-based industry in development of environment-friendly processes and products, an optimal toolbox of biocatalysts is key. Although functional screen of (meta)genomic libraries may potentially contribute to identifying new enzymes, the discovery of new enzymes meeting industry compliance demands is still challenging. This is particularly noticeable in the case of proteases, for which the reports of metagenome-derived proteases with industrial applicability are surprisingly limited. Indeed, proteolytic clones have been typically assessed by its sole activity on casein or skim milk and limited to mild screening conditions. Here, we demonstrate the use of six industry-relevant animal and plant by-products, namely bone, feather, blood meals, gelatin, gluten, and zein, as compl

SUBMITTER: Garcia-Moyano A 

PROVIDER: S-EPMC8053189 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature