Production of nonulosonic acids in the extracellular polymeric substances of "Candidatus Accumulibacter phosphatis".

ABSTRACT: Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular matrix of virtually all microbial life and in "Candidatus Accumulibacter phosphatis", a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs pres

SUBMITTER: Tomas-Martinez S 

PROVIDER: S-EPMC8053191 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature