ΔNp63 is a pioneer factor that binds inaccessible chromatin and elicits chromatin remodeling.

ABSTRACT:

Background

ΔNp63 is a master transcriptional regulator playing critical roles in epidermal development and other cellular processes. Recent studies suggest that ΔNp63 functions as a pioneer factor that can target its binding sites within inaccessible chromatin and induce chromatin remodeling.

Methods

In order to examine if ΔNp63 can bind to inaccessible chromatin and to determine if specific histone modifications are required for binding, we induced ΔNp63 expression in two p63-naïve cell lines. ΔNp63 binding was then examined by ChIP-seq and the chromatin at ΔNp63 targets sites was examined before and after binding. Further analysis with competitive nucleosome binding assays was used to determine how ΔNp63 directly interacts with nucleosomes.

Results

Our results show

SUBMITTER: Yu X 

PROVIDER: S-EPMC8053304 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature