An Ion Chromatography-Ultrahigh-Resolution-MS¹/Data-Independent High-Resolution MS² Method for Stable Isotope-Resolved Metabolomics Reconstruction of Central Metabolic Networks.

ABSTRACT: The metabolome comprises a complex network of interconnecting enzyme-catalyzed reactions that involve transfers of numerous molecular subunits. Thus, the reconstruction of metabolic networks requires metabolite substructures to be tracked. Subunit tracking can be achieved by tracing stable isotopes through metabolic transformations using NMR and ultrahigh -resolution (UHR)-mass spectrometry (MS). UHR-MS¹ readily resolves and counts isotopic labels in metabolites but requires tandem MS to help identify isotopic enrichment in substructures. However, it is challenging to perform chromatography-based UHR-MS¹ with its long acquisition time, while acquiring MS² data on many coeluting labeled isotopologues for each metabolite. We have developed an ion chromatograp

SUBMITTER: Sun Q 

PROVIDER: S-EPMC8053425 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature