Defining vitamin D receptor expression in the brain using a novel VDR^Cre mouse.

ABSTRACT: Vitamin D action has been linked to several diseases regulated by the brain including obesity, diabetes, autism, and Parkinson's. However, the location of the vitamin D receptor (VDR) in the brain is not clear due to conflicting reports. We found that two antibodies previously published as specific in peripheral tissues are not specific in the brain. We thus created a new knockin mouse with cre recombinase expression under the control of the endogenous VDR promoter (VDR^Cre ). We demonstrated that the cre activity in the VDR^Cre mouse brain (as reported by a cre-dependent tdTomato expression) is highly overlapping with endogenous VDR mRNAs. These VDR-expressing cells were enriched in multiple brain regions including the cortex, amygdala, caudate putamen, and hypothalamu

SUBMITTER: Liu H 

PROVIDER: S-EPMC8053677 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature