Hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G₁/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.

ABSTRACT: We identified a novel interactome, circ_0001018/miR-338-3p/SOX4, in papillary thyroid cancer (PTC), and we intended to confirm the regulatory relationship between the three and to study the effects of the three in PTC. The bioinformatics method was used to screen out the circular RNA and mRNA of interest. A cellular fractionation assay and fluorescence in situ hybridization (FISH) assay were conducted to prove that circ_0001018 and CCT4 (the host gene of circ_0001018) mRNA primarily localized in the cytoplasm of PTC cell lines. By qRT-PCR analysis, the expression of circ_0001018 and SOX4 mRNA was found upregulated while the expression of miR-338-3p was found downregulated in PTC tissues and cells. circ_0001018 silence significantly inhibited the tumor growth in xenografted nude mice

SUBMITTER: Luo Q 

PROVIDER: S-EPMC8054110 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature