Comparative Analysis of One-Dimensional Protein Fingerprint by Line Shape Enhancement and Two-Dimensional ¹H,¹³C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.

ABSTRACT: The use of NMR spectroscopy has emerged as a premier tool to characterize the higher order structure of protein therapeutics and in particular IgG1 monoclonal antibodies (mAbs). Due to their large size, traditional ¹H-¹⁵N correlation experiments have proven exceedingly difficult to implement on mAbs, and a number of alternative techniques have been proposed, including the one-dimensional (1D) ¹H protein fingerprint by line shape enhancement (PROFILE) method and the two-dimensional (2D) ¹H-¹³C methyl correlation-based approach. Both 1D and 2D approaches have relative strengths and weaknesses, related to the inherent sensitivity and resolution of the respective methods. To further increase the utility of NMR to the biopharmaceutical comm

SUBMITTER: Elliott KW 

PROVIDER: S-EPMC8054654 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature