Changes in NAD and Lipid Metabolism Drive Acidosis-Induced Acute Kidney Injury.

ABSTRACT:

Background

The kidney plays an important role in maintaining normal blood pH. Metabolic acidosis (MA) upregulates the pathway that mitochondria in the proximal tubule (PT) use to produce ammonia and bicarbonate from glutamine, and is associated with AKI. However, the extent to which MA causes AKI, and thus whether treating MA would be beneficial, is unclear.

Methods

Gavage with ammonium chloride induced acute MA. Multiphoton imaging of mitochondria (NADH/membrane potential) and transport function (dextran/albumin uptake), oxygen consumption rate (OCR) measurements in isolated tubules, histologic analysis, and electron microscopy in fixed tissue, and urinary biomarkers (KIM-1/clara cell 16) assessed tubular cell structure and function in mouse kidney cortex.

Results

MA

SUBMITTER: Bugarski M 

PROVIDER: S-EPMC8054907 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature