High-yield purification of exceptional-quality, single-molecule DNA substrates.

ABSTRACT: Single-molecule studies involving DNA or RNA, require homogeneous preparations of nucleic acid substrates of exceptional quality. Over the past several years, a variety of methods have been published describing different purification methods but these are frustratingly inconsistent with variable yields even in the hands of experienced bench scientists. To address these issues, we present an optimized and straightforward, column-based approach that is reproducible and produces high yields of substrates or substrate components of exceptional quality. Central to the success of the method presented is the use of a non-porous anion exchange resin. In addition to the use of this resin, we encourage the optimization of each step in the construction of substrates. The fully optimized method produc

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8054919 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature