Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain.

ABSTRACT: Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM.

SUBMITTER: Dan D 

PROVIDER: S-EPMC8054977 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature