Transcriptional characterisation of human lung cells identifies novel mesenchymal lineage markers.

ABSTRACT:

Rationale

The lung mesenchyme gives rise to multiple distinct lineages of cells in the mature respiratory system, including smooth muscle cells of the airway and vasculature. However, a thorough understanding of the specification and mesenchymal cell diversity in the human lung is lacking.

Methods

We completed single-cell RNA sequencing analysis of fetal human lung tissues. Canonical correlation analysis, clustering, cluster marker gene identification and t-distributed stochastic neighbour embedding representation was performed in Seurat. Cell populations were annotated using ToppFun. Immunohistochemistry and in situ hybridisation were used to validate spatiotemporal gene expression patterns for key marker genes.

Results

We identified molecularly distinct popul

SUBMITTER: Danopoulos S 

PROVIDER: S-EPMC8055172 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature