Evaluation of an oxygen-dependent self-inducible surfactin synthesis in B. subtilis by substitution of native promoter P_srfA by anaerobically active P_narG and P_nasD.

ABSTRACT: A novel approach targeting self-inducible surfactin synthesis under oxygen-limited conditions is presented. Because both the nitrate (NarGHI) and nitrite (NasDE) reductase are highly expressed during anaerobic growth of B. subtilis, the native promoter P_srfA of the surfactin operon in strain B. subtilis JABs24 was replaced by promoters P_narG and P_nasD to induce surfactin synthesis anaerobically. Shake flask cultivations with varying oxygen availabilities indicated no significant differences in native P_srfA expression. As hypothesized, activity of P_narG and P_nasD increased with lower oxygen levels and surfactin was not produced by P_srfA::P_narG as well as P_srfA::P_nasD mutant strains

SUBMITTER: Hoffmann M 

PROVIDER: S-EPMC8055807 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature