Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes.

ABSTRACT: High-throughput nucleic acid sequencing has greatly accelerated the discovery of viruses in the environment. Mosquitoes, because of their public health importance, are among those organisms whose viromes are being intensively characterized. Despite the deluge of sequence information, our understanding of the major drivers influencing the ecology of mosquito viromes remains limited. Using methods to increase the relative proportion of microbial RNA coupled with RNA-seq we characterize RNA viruses and other symbionts of three mosquito species collected along a rural to urban habitat gradient in Thailand. The full factorial study design allows us to explicitly investigate the relative importance of host species and habitat in structuring viral communities. We found that the pattern of virus p

SUBMITTER: Thongsripong P 

PROVIDER: S-EPMC8055903 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature