Molecular characterization and transcriptional regulation of two types of H⁺-pyrophosphatases in the scuticociliate parasite Philasterides dicentrarchi.

ABSTRACT: Proton-translocating inorganic pyrophosphatases (H⁺-PPases) are an ancient family of membrane bound enzymes that couple pyrophosphate (PPi) hydrolysis to H⁺ translocation across membranes. In this study, we conducted a molecular characterization of two isoenzymes (PdVP1 and PdVP2) located in respectively the alveolar sacs and in the membranes of the intracellular vacuoles of a scuticociliate parasite (Philasterides dicentrarchi) of farmed turbot. We analyzed the genetic expression of the isoenzymes after administration of antiparasitic drugs and after infection in the host. PdVP1 and PdVP2 are encoded by two genes of 2485 and 3069 bp, which respectively contain 3 and 11 exons and express proteins of 746 and 810 aa of molecular mass 78.9 and 87.6 kDa. Topological predi

SUBMITTER: Folgueira I 

PROVIDER: S-EPMC8055999 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature