Performance evaluation of five lipoprotein(a) immunoassays on the Roche cobas c501 chemistry analyzer.

ABSTRACT:

Objectives

Measurement of lipoprotein(a) [Lp(a)] is used in risk assessment of atherosclerotic cardiovascular disease (ASCVD). The aim of the current study was to evaluate performance characteristic of five different Lp(a) assays using the cobas c501 (Roche Diagnostics) analyzer.

Design and methods

Lp(a) was measured using five Lp(a) assays (Diazyme, Kamiya, MedTest, Randox, and Roche) configured to mg/dL units. Assays from Diazyme and Kamiya were also configured using nmol/L units in separate experiments. Studies included sensitivity, imprecision, linearity, method comparison, and evaluation of healthy subjects. Imprecision (intra-day, 20 replicates; inter-day, duplicates twice daily for five days) and linearity were evaluated using patient pools. Linearity assessed a minim

SUBMITTER: Wyness SP 

PROVIDER: S-EPMC8056269 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature