Growth factor profile in calcified cartilage from the metaphysis of a calf costochondral junction, the site of initial bone formation.

ABSTRACT: Endochondral bone formation is orchestrated by growth factors produced by chondrocytes and deposited in the cartilage matrix. Whilst some of these factors have been identified, the complete list and their relationship remains unknown. In the present study, the growth factors were isolated from non-calcified and calcified cartilage of costochondral junctions. Cartilage dissected from the ribs of 6-20-week-old calves was purchased from a local butcher within 24 h of the death of the animal. The isolation involved hyaluronidase digestion, guanidinium hydrochloride (GuHCl) extraction, HCl decalcification and GuHCl extraction of the decalcified matrix. Growth factors were purified by heparin chromatography and their quantities were estimated using ELISA. Decalcified cartilage was also used for

SUBMITTER: Iwan A 

PROVIDER: S-EPMC8056382 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature