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Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies.

ABSTRACT: There is growing interest in the characterization of protein complexes and their interactions with ligands using native ion mobility mass spectrometry. A particular challenge, especially for membrane proteins, is preserving noncovalent interactions and maintaining native-like structures. Different approaches have been developed to minimize activation of protein complexes by manipulating charge on protein complexes in solution and the gas-phase. Here, we report the utility of polyamines that have exceptionally high charge-reducing potencies with some molecules requiring 5-fold less than trimethylamine oxide to elicit the same effect. The charge-reducing molecules do not adduct to membrane protein complexes and are also compatible with ion-mobility mass spectrometry, paving the way for improved methods of charge reduction.

SUBMITTER: Lyu J

PROVIDER: S-EPMC8056390 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies.

Lyu Jixing J Liu Yang Y McCabe Jacob W JW Schrecke Samantha S Fang Lei L Russell David H DH Laganowsky Arthur A

Analytical chemistry 20200731 16

There is growing interest in the characterization of protein complexes and their interactions with ligands using native ion mobility mass spectrometry. A particular challenge, especially for membrane proteins, is preserving noncovalent interactions and maintaining native-like structures. Different approaches have been developed to minimize activation of protein complexes by manipulating charge on protein complexes in solution and the gas-phase. Here, we report the utility of polyamines that have ...[more]

PMID: 32672445

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