Cocaine reward and memory after chemogenetic inhibition of distinct serotonin neuron subtypes in mice.

ABSTRACT:

Rationale

We probed serotonin neurons, those denoted by their developmental gene expression as r2Hoxa2-Pet1 (experiment 1) and Drd1a-Pet1 (experiment 2), for differential modulation of cocaine reward and memory as revealed by the expression and development of conditioned place preference (CPP) in transgenic mice.

Objectives

To query roles in CPP, we inhibited neurons cell autonomously in vivo by activating the transgenically expressed, synthetic DREADD receptor hM₄Di (Di) with the exogenous ligand clozapine-N-oxide (CNO).

Methods

To examine CPP expression, mice were conditioned using behaviorally active doses of cocaine (10.0 or 17.8 mg/kg) vs. saline followed by CPP assessment, first without neuron inhibition (post-conditioning session 1), and then with CN

SUBMITTER: Baskin BM 

PROVIDER: S-EPMC8057056 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature