Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays.

ABSTRACT: The DNA double strand breaks (DSBs) created during meiotic recombination and during some types of chemotherapy contain protein covalently attached to their 5' termini. Removal of the end-blocking protein is a prerequisite to DSB processing by non-homologous end-joining or homologous recombination. One mechanism for removing the protein involves CtIP-stimulated Mre11-catalyzed nicking of the protein-linked strand distal to the DSB terminus, releasing the end-blocking protein while it remains covalently attached to an oligonucleotide. Much of what is known about this repair process has recently been deciphered through in vitro reconstitution studies. We present here a novel model system based on adenovirus (Ad), which contains the Ad terminal protein covalently linked to the 5' terminus of i

SUBMITTER: Lamarche BJ 

PROVIDER: S-EPMC8057058 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature