Characterization and functional analysis of the proteins Prohibitin 1 and 2 in Trypanosoma cruzi.

ABSTRACT:

Background

Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. cruzi PHBs revealed a pleiotropic function in different stages of the parasite, participating actively in the transformation of the non-infective replicative epimastigote form into metacyclic trypomastigotes and also in the multiplication of intracellular amastigotes.

Methodology/principal findings

To obtain and confirm our results, we applied several tools and techniques such as electron microscopy, immuno-electron microscopy, bioinformatics analysis and molecular biology. We transfected T. cruzi clones with the PHB genes, in order to ov

SUBMITTER: Ibarrola-Vannucci AK 

PROVIDER: S-EPMC8057595 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature