Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.
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ABSTRACT:
SUBMITTER: Yang Q
PROVIDER: S-EPMC8057811 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.
Yang Qing Q Meyerson Nicholas R NR Clark Stephen K SK Paige Camille L CL Fattor Will T WT Gilchrist Alison R AR Barbachano-Guerrero Arturo A Healy Benjamin G BG Worden-Sapper Emma R ER Wu Sharon S SS Muhlrad Denise D Decker Carolyn J CJ Saldi Tassa K TK Lasda Erika E Gonzales Patrick P Fink Morgan R MR Tat Kimngan L KL Hager Cole R CR Davis Jack C JC Ozeroff Christopher D CD Brisson Gloria R GR McQueen Matthew B MB Leinwand Leslie A LA Parker Roy R Sawyer Sara L SL
eLife 20210329
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols t ...[more]
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