Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts.

ABSTRACT:

Key message

Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Nicotiana benthamiana is widely used for agroinfiltration and the characterisation of broad-spectrum effectors. The method has also been used in other plant species including field pea, but not yet developed for chickpea, lentil, or faba bean. Here, we have modified the pEAQ-HT-D

SUBMITTER: Debler JW 

PROVIDER: S-EPMC8058004 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature