Production and properties of non-cytotoxic pyomelanin by laccase and comparison to bacterial and synthetic pigments.

ABSTRACT: Pyomelanin is a polymer of homogentisic acid synthesized by microorganisms. This work aimed to develop a production process and evaluate the quality of the pigment. Three procedures have been elaborated and optimized, (1) an HGA-Mn²⁺ chemical autoxidation (Pyo_CHEM yield 0.317 g/g substrate), (2) an induced bacterial culture of Halomonas titanicae through the 4-hydroxyphenylacetic acid-1-hydroxylase route (Pyo_BACT, 0.55 g/L), and (3) a process using a recombinant laccase extract with the highest level produced (Pyo_ENZ, 1.25 g/g substrate) and all the criteria for a large-scale prototype. The chemical structures had been investigated by ¹³C solid-state NMR (CP-MAS) and FTIR. C_ar-C_ar bindings predominated in the thre

SUBMITTER: Lorquin F 

PROVIDER: S-EPMC8058095 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature