Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions.

ABSTRACT: Age-related macular degeneration (AMD) is a highly prevalent form of blindness caused by loss death of cells of the retinal pigment epithelium (RPE). Transplantation of pluripotent stem cell (PSC)-derived RPE cells is considered a promising therapy to regenerate cell function and vision.

Objective

The objective of this study is to develop a rapid directed differentiation method for production of RPE cells from PSC which is rapid, efficient, and fully defined and produces cells suitable for clinical use.

Design

A protocol for cell growth and differentiation from hESCs was developed to induce differentiation through screening small molecules which regulated a primary stage of differentiation to the eyefield progenitor, and then, a subsequent set of molecules to drive differenti

SUBMITTER: Limnios IJ 

PROVIDER: S-EPMC8058973 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature