Regulation of osteoclastogenesis by mast cell in rheumatoid arthritis.

ABSTRACT:

Background

In the pathogenesis of rheumatoid arthritis (RA), the role of mast cells has not been revealed clearly. We aimed to define the inflammatory and tissue-destructive roles of mast cells in rheumatoid arthritis (RA).

Methods

Serum and synovial fluid (SF) concentration levels of tryptase, chymase, and histamine were quantified using ELISA. After activating mast cells using IL-33, the production of TNF-α, IL-1β, IL-6, IL-17, RANKL, and MMPs was determined using real-time PCR and ELISA. Osteoclastogenesis was assessed in CD14+ monocytes from peripheral blood and SF, which were cultured with IL-33-activated mast cells, by counting TRAP-positive multinucleated cells.

Results

The concentration levels of serum tryptase, chymase, and histamine and SF histamine were hig

SUBMITTER: Kim KW 

PROVIDER: S-EPMC8059019 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature