Establishment of functional epithelial organoids from human lacrimal glands.

ABSTRACT:

Background

Tear deficiency due to lacrimal gland (LG) dysfunction is one of the major causes of dry eye disease (DED). Therefore, LG stem cell-based therapies have been extensively reported to regenerate injured lacrimal tissue; however, the number of stem cells in the LG tissue is low, and 2D long-term cultivation reduces the differentiation capacity of stem cells. Nevertheless, 3D LG organoids could be an alternative for a DED therapy because it is capable of prolonged growth while maintaining the characteristics of the LG tissue. Here, we report the development of LG organoids and their application as cell therapeutics.

Methods

Digested cells from human LG tissue were mixed with Matrigel and cultured in five different media modified from human prostate/salivary organoid c

SUBMITTER: Jeong SY 

PROVIDER: S-EPMC8059179 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature