The factor XIII-A Val34Leu polymorphism decreases whole blood clot mass at high fibrinogen concentrations.

ABSTRACT:

Background

Factor XIII (FXIII) promotes fibrin crosslinking and red blood cell (RBC) retention in clots. The FXIII-A polymorphism, Val34Leu, is associated with protection against venous thrombosis. This effect is hypothesized to result from fibrinogen concentration-dependent changes in fibrin structure. Effects of the FXIII-A Val34Leu polymorphism in whole blood clots have not been investigated.

Aim

Characterize effects of FXIII-A Val34Leu polymorphism and fibrinogen on whole blood clots.

Methods

We isolated platelet-poor plasmas from human donors (FXIII^Val/Val , FXIII^Val/Leu , FXIII^Leu/Leu ), reconstituted plasmas with platelets and RBCs, and triggered clotting. We assessed contributions of gender, age, clotting times, thrombin genera

SUBMITTER: Kattula S 

PROVIDER: S-EPMC8059250 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature