Clinical Utility of Droplet Digital PCR to Monitor BCR-ABL1 Transcripts of Patients With Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia Post-chimeric Antigen Receptor19/22 T-Cell Cocktail Therapy.

ABSTRACT: Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph⁺ ALL) accounts for 20-30% of adult patients with ALL, characterized by translocation of t _{(9, 22)}. Tyrosine kinase inhibitors (TKIs) have significantly improved the outcome even though there are still some problems including relapse due to drug-resistant mutations and suboptimal molecular remission depth. Previously, we reported the safety and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy in the treatment of relapsed/refractory (R/R) B-cell neoplasms including cases with Ph⁺ ALL. Given possible deeper reaction, more patients were expected to reach optimal minimal residual disease (MRD) response. An alternative method, duplex droplet d

SUBMITTER: Guan Y 

PROVIDER: S-EPMC8059437 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature