Reactivity of human antisera to codon optimized SARS-CoV2 viral proteins expressed in Escherichia coli.

ABSTRACT:

Objective

The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV2 virus continues to pose a serious threat to public health worldwide. The development of rapid diagnostic kits can assist the Tzu Chi Foundation in supporting global volunteers working to provide relief during the current pandemic.

Materials and methods

In this study, nucleotide sequences derived from publicly available viral genome data for several domains of the SARS-CoV2 spike and nucleocapsid (N) proteins were chemically synthesized, with codon optimization for Escherichia coli protein expression. No actual viral particles were involved in these experiments. The synthesized sequences were cloned into an E. coli expression system based on pQE80L, and expressed viral proteins we

SUBMITTER: Toh YH 

PROVIDER: S-EPMC8059472 | biostudies-literature | 2021 Apr-Jun

REPOSITORIES: biostudies-literature