Development and validation of point-of-care testing of albuminuria for early screening of chronic kidney disease.

ABSTRACT:

Introduction

Chronic kidney disease (CKD) is a significant global health issue. As the prevalence of renal replacement therapy (RRT) in Thailand is increasing, early detection and management of CKD is the most important step to prevent CKD progression and the need for RRT. Current diagnostic tests for CKD are non-specific and expensive. We aimed to develop and validate antibody-based-albumin point-of-care testing (POCT) to detect patients with impaired kidney function at early stage.

Methods

The prototype strip test was developed under the concept of competitive lateral flow immunochromatography assay, or strip test. Monoclonal antibodies (MAbs) to human serum albumin (HSA) were harvested from the hybridomas of spleen cells from immunized mice and mouse myeloma cells. Presen

SUBMITTER: Vutthikraivit N 

PROVIDER: S-EPMC8059747 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature