High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns.

ABSTRACT: The recent discovery of the mode of action of the CRISPR/Cas9 system has provided biologists with a useful tool for generating site-specific mutations in genes of interest. In plants, site-targeted mutations are usually obtained by the stable transformation of a Cas9 expression construct into the plant genome. The efficiency of introducing mutations in genes of interest can vary considerably depending on the specific features of the constructs, including the source and nature of the promoters and terminators used for the expression of the Cas9 gene and the guide RNA, and the sequence of the Cas9 nuclease itself. To optimize the efficiency of the Cas9 nuclease in generating mutations in target genes in Arabidopsis thaliana, we investigated several features of its nucleotide and/or am

SUBMITTER: Grutzner R 

PROVIDER: S-EPMC8060730 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature