Selective ¹H-¹⁴N Distance Measurements by ¹⁴N Overtone Solid-State NMR Spectroscopy at Fast MAS.

ABSTRACT: Accurate distance measurements between proton and nitrogen can provide detailed information on the structures and dynamics of various molecules. The combination of broadband phase-modulated (PM) pulse and rotational-echo saturation-pulse double-resonance (RESPDOR) sequence at fast magic-angle spinning (MAS) has enabled the measurement of multiple ¹H-¹⁴N distances with high accuracy. However, complications may arise when applying this sequence to systems with multiple inequivalent ¹⁴N nuclei, especially a single ¹H sitting close to multiple ¹⁴N atoms. Due to its broadband characteristics, the PM pulse saturates all ¹⁴N atoms; hence, the single ¹H simultaneously experiences the RESPDOR effect from multiple ¹

SUBMITTER: Duong NT 

PROVIDER: S-EPMC8061749 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature