Master Blaster: an approach to sensitive identification of remotely related proteins.

ABSTRACT: Genome sequencing projects unearth sequences of all the protein sequences encoded in a genome. As the first step, homology detection is employed to obtain clues to structure and function of these proteins. However, high evolutionary divergence between homologous proteins challenges our ability to detect distant relationships. In the past, an approach involving multiple Position Specific Scoring Matrices (PSSMs) was found to be more effective than traditional single PSSMs. Cascaded search is another successful approach where hits of a search are queried to detect more homologues. We propose a protocol, 'Master Blaster', which combines the principles adopted in these two approaches to enhance our ability to detect remote homologues even further. Assessment of the approach was performed using

SUBMITTER: Janaki C 

PROVIDER: S-EPMC8062480 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature