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The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

ABSTRACT: The infraorder Brachyura (true or short-tailed crabs) represents a successful group of marine invertebrates yet with limited genomic resources. Here we report a chromosome-anchored reference genome and transcriptomes of the Chinese mitten crab Eriocheir sinensis, a catadromous crab and invasive species with wide environmental tolerance, strong osmoregulatory capacity and high fertility. We show the expansion of specific gene families in the crab, including F-ATPase, which enhances our knowledge on the adaptive plasticity of this successful invasive species. Our analysis of spatio-temporal transcriptomes and the genome of E. sinensis and other decapods shows that brachyurization development is associated with down-regulation of Hox genes at the megalopa stage when tail shortening occurs. A better understanding of the molecular mechanism regulating sexual development is achieved by integrated analysis of multiple omics. These genomic resources significantly expand the gene repertoire of Brachyura, and provide insights into the biology of this group, and Crustacea in general.

SUBMITTER: Cui Z

PROVIDER: S-EPMC8062507 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

Cui Zhaoxia Z Liu Yuan Y Yuan Jianbo J Zhang Xiaojun X Ventura Tomer T Ma Ka Yan KY Sun Shuai S Song Chengwen C Zhan Dongliang D Yang Yanan Y Liu Hourong H Fan Guangyi G Cai Qingle Q Du Jing J Qin Jing J Shi Chengcheng C Hao Shijie S Fitzgibbon Quinn P QP Smith Gregory G GG Xiang Jianhai J Chan Tin-Yam TY Hui Min M Bao Chenchang C Li Fuhua F Chu Ka Hou KH

Nature communications 20210422 1

The infraorder Brachyura (true or short-tailed crabs) represents a successful group of marine invertebrates yet with limited genomic resources. Here we report a chromosome-anchored reference genome and transcriptomes of the Chinese mitten crab Eriocheir sinensis, a catadromous crab and invasive species with wide environmental tolerance, strong osmoregulatory capacity and high fertility. We show the expansion of specific gene families in the crab, including F-ATPase, which enhances our knowledge ...[more]

PMID: 33888695

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Project description:Chinese mitten crab (Eriocheir sinensis) is an economically important freshwater aquaculture species and is a model species for research on the mechanism of molting. This study aimed to identify important candidate genes associated with the molting process and to determine the role of gills in the regulation of molting with the help of transcriptomic analysis. The transcriptomes of crabs at different molting stages-postmolt (PoM), intermolt (InM), premolt (PrM) and ecdysis (E)-were de novo assembled to generate 246,232 unigenes with a mean length of 851 bp. A total of 86,634 unigenes (35.18% of the total unigenes) were annotated against reference databases. Significantly upregulated genes were identified in postmolt compared to intermolt (1,475), intermolt compared to premolt (65), premolt compared to ecdysis (1,352), and ecdysis compared to postmolt (153), and the corresponding numbers of downregulated genes were 1,276, 32, 1,573 and 171, respectively. Chitin synthase, endochitinase, chitinase A, chitinase 3, chitinase 6 and chitin deacetylase 1 were upregulated during the postmolt and ecdysis stages, while phosphoglucomutase 3 (PGM3), glucosamine 6-phosphate deaminase (GNPDA) and glucosamine glycoside hydrolase (nagZ) were upregulated during the intermolt and premolt stages compared to the other stages. The upregulated genes were enriched in several lipid-related metabolic pathways, such as "fatty acid elongation", "glycerophospholipid metabolism" and "sulfur metabolism". Meanwhile, three signaling pathways, including the "phosphatidylinositol signaling system", the "calcium signaling pathway" and the "GnRH signaling pathway" were also enriched. Tetraspanin-18, an important effector gene in the lysosomal pathway involved in cell apoptosis, up-regulate with the beginning of molting (in premolt stage) and reach the top in the ecdysis stage, and barely expressed in the intermolt stage. The expression variations in the tetraspanin-18 gene indicated that it may play an important role in the beginning of molting cycle, which might be regulated by the stress of salinity. This study revealed that the gills could participate in chitin degradation, in reestablishment of the exoskeleton and the signaling process. Based on transcriptomic analysis of the gills, we not only explored novel molecular mechanisms of molting in E. sinensis but also acquired foundational genetic data for E. sinensis.

Dataset Information

The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

Publications

The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

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