Enzymes of an alternative pathway of glucose metabolism in obligate methanotrophs.

ABSTRACT: Aerobic methanotrophic bacteria utilize methane as a growth substrate but are unable to grow on any sugars. In this study we have shown that two obligate methanotrophs, Methylotuvimicrobium alcaliphilum 20Z and Methylobacter luteus IMV-B-3098, possess functional glucose dehydrogenase (GDH) and gluconate kinase (GntK). The recombinant GDHs from both methanotrophs were homotetrameric and strongly specific for glucose preferring NAD⁺ over NADP⁺. GDH from Mtm. alcaliphilum was most active at pH 10 (V_max = 95 U/mg protein) and demonstrated very high K_m for glucose (91.8 ± 3.8 mM). GDH from Mb. luteus was most active at pH 8.5 (V_max = 43 U/mg protein) and had lower K_m for glucose (16 ± 0.6 mM). The cells of two Mtm. alcaliphilum d

SUBMITTER: Rozova ON 

PROVIDER: S-EPMC8062543 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature