Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS³: The Effects of Bioprocessing Conditions on Productivity and Product Quality.

ABSTRACT: The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in combination with optimization of various process parameters results in increasing product titers. To enable further improvements in manufacturing processes, detailed information about how certain parameters affect cellular mechanisms in the production cells, and thereby also the expressed drug substance, is required. Therefore, in this study the effects of commonly applied changes in bioprocessing parameters on an anti-IL8 IgG1 producing CHO DP-12 cell line were investigated on the level of host cell proteome expression combined with product quality assessment of the expressed IgG1 monoclonal antibody. Applying shifts

SUBMITTER: Strasser L 

PROVIDER: S-EPMC8062983 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature