Decreased Levels of STAT1 and Interferon-γ-Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells.

ABSTRACT:

Objective

We investigated whether a previously reported association of IFNGR expression with rheumatoid arthritis (RA) and its radiographic severity reflects differences in proximal interferon-γ (IFN-γ) signaling in T cells from patients with RA compared with healthy controls (HC).

Methods

Using phosphoflow cytometry, we compared IFN-γ-stimulated signal transducer and activator of transcription 1 (STAT1) activation in CD4⁺ and CD8⁺ T-cell populations from patients with RA and HC.

Results

Compared with controls, patients with RA had a higher proportion of CD4⁺ T cells, associated with expansion of the CD4⁺ effector memory subset. Several CD4⁺ T-cell types exhibited reduced IFN-γ-induced phosphoSTAT1^Y701

SUBMITTER: Sharma V 

PROVIDER: S-EPMC8063148 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature