Cathelicidin antimicrobial peptide (CAMP) gene promoter methylation induces chondrocyte apoptosis.

ABSTRACT:

Objective

The occurrence of osteoarthritis is related to genetic and environmental factors. Among them, the change of chondrocyte gene expression pattern regulated by epigenetic modification is an important participant. This study analyzed the effect of CAMP gene methylation on the level of oxidative stress and inflammation of chondrocytes.

Methods

We analyzed the changes of the transcriptome in the articular cartilage tissue of osteoarthritis (OA) patients from the GSE117999 dataset. The GSE48422 dataset was used to analyze the changes in the methylation level of osteoarthritis cells. Cell Counting Kit-8 (CCK-8) and flow cytometry analysis of short hairpin RNA (shRNA) silencing CAMP gene and 5-μM 5-Aza-2'-Deoxycytidine (AZA) treatment on the proliferation and apoptosis of H

SUBMITTER: Wang G 

PROVIDER: S-EPMC8063489 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature