Inducing different severities of traumatic brain injury in Drosophila using a piezoelectric actuator.

ABSTRACT: Drosophila models have been instrumental in providing insights into molecular mechanisms of neurodegeneration, with wide application to human disease. The brain degeneration associated with traumatic brain injury (TBI) has been modeled in Drosophila using devices that inflict trauma on multiple parts of the fly body, including the head. However, the injuries produced by these models are not specific in location and are inconsistent between individual animals. We have recently developed a device that can be used to inflict controlled head injury to flies, resulting in physiological responses that are remarkably similar to those observed in humans with TBI. This protocol describes the construction, calibration and use of the Drosophila TBI (dTBI) device, a platform that employs a piezoelectr

SUBMITTER: Saikumar J 

PROVIDER: S-EPMC8063611 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature