A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system.
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ABSTRACT:
SUBMITTER: He L
PROVIDER: S-EPMC8064019 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
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A protocol for <i>in vivo</i> analysis of liver tumorigenesis in mice using sleeping beauty transposon system.
He Lingli L Yu Wentao W Lu Yi Y Zhang Wenxiang W Xu Jinjin J Zhang Lei L
STAR protocols 20210410 2
Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an <i>in vivo</i> liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop n ...[more]
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