Sodium activates human monocytes via the NADPH oxidase and isolevuglandin formation.

ABSTRACT:

Aims

Prior studies have focused on the role of the kidney and vasculature in salt-induced modulation of blood pressure; however, recent data indicate that sodium accumulates in tissues and can activate immune cells. We sought to examine mechanisms by which salt causes activation of human monocytes both in vivo and in vitro.

Methods and results

To study the effect of salt in human monocytes, monocytes were isolated from volunteers to perform several in vitro experiments. Exposure of human monocytes to elevated Na+ex vivo caused a co-ordinated response involving isolevuglandin (IsoLG)-adduct formation, acquisition of a dendritic cell (DC)-like morphology, expression of activation markers CD83 and CD16, and increased production of pro-inflammatory cytokines tumour necrosis fact

SUBMITTER: Ruggeri Barbaro N 

PROVIDER: S-EPMC8064439 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature