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Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility.

ABSTRACT: Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the aggregates growth and internal cell motility, while selective compressions of same magnitude have almost no effect. Both compressions alter the volume of individual cells in the same way over a shor-timescale, but, by draining the water out of the extracellular matrix, the global one imposes a residual compressive mechanical stress on the cells over a long-timescale, while the selective one does not. We conclude that the extracellular matrix is as a sensor that mechanically regulates cell proliferation and migration in a 3D environment.

SUBMITTER: Dolega ME

PROVIDER: S-EPMC8064752 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility.

Dolega Monika E ME Monnier Sylvain S Brunel Benjamin B Joanny Jean-François JF Recho Pierre P Cappello Giovanni G

eLife 20210311

Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the a ...[more]

PMID: 33704063

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Dataset Information

Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility.

Publications

Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility.

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