Purification and quantitative proteomic analysis of cell bodies and protrusions.
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ABSTRACT:
SUBMITTER: Dermit M
PROVIDER: S-EPMC8065254 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
Purification and quantitative proteomic analysis of cell bodies and protrusions.
Dermit Maria M Mardakheh Faraz K FK
STAR protocols 20210410 2
Actin-rich protrusions are membrane extensions generated by actin polymerization that drive mesenchymal-like cell migration. Characterization of protrusions proteome is crucial for understanding their function. We present a complete step-by-step protocol based on microporous filter-based fractionation of protrusive cellular domains coupled with sample preparation for quantitative proteomics, mass spectrometric data acquisition, and data analysis. This protocol enables purification, quantificatio ...[more]
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