Can Vitamin B12 Assist the Internalization of Antisense LNA Oligonucleotides into Bacteria?

ABSTRACT: The emergence of bacterial resistance to traditional small-molecule antibiotics is fueling the search for innovative strategies to treat infections. Inhibiting the expression of essential bacterial genes using antisense oligonucleotides (ASOs), particularly composed of nucleic acid mimics (NAMs), has emerged as a promising strategy. However, their efficiency depends on their association with vectors that can translocate the bacterial envelope. Vitamin B12 is among the largest molecules known to be taken up by bacteria and has very recently started to gain interest as a trojan-horse vector. Gapmers and steric blockers were evaluated as ASOs against Escherichia coli (E. coli). Both ASOs were successfully conjugated to B12 by copper-free azide-alkyne click-chemistry. The biological effect of

SUBMITTER: Pereira S 

PROVIDER: S-EPMC8065541 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature