Next-Generation Sequencing Identifies Potential Actionable Targets in Paediatric Sarcomas.

ABSTRACT: Background: Bone and soft-tissue sarcomas represent 13% of all paediatric malignancies. International contributions to introduce next-generation sequencing (NGS) approaches into clinical application are currently developing. We present the results from the Precision Medicine program for children with sarcomas at a reference centre. Results: Samples of 70 paediatric sarcomas were processed for histopathological analysis, reverse transcriptase polymerase chain reaction (RT-PCR) and next-generation sequencing (NGS) with a consensus gene panel. Pathogenic alterations were reported and, if existing, targeted recommendations were translated to the clinic. Seventy paediatric patients with sarcomas from 10 centres were studied. Median age was 11.5 years (range 1-18). Twenty-two (31%) had at least

SUBMITTER: Juan Ribelles A 

PROVIDER: S-EPMC8067272 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature